DNA methylation defines regional identity of human intestinal epithelial organoids and undergoes dynamic changes during development.

ABSTRACT:

Objective

Human intestinal epithelial organoids (IEOs) are increasingly being recognised as a highly promising translational research tool. However, our understanding of their epigenetic molecular characteristics and behaviour in culture remains limited.

Design

We performed genome-wide DNA methylation and transcriptomic profiling of human IEOs derived from paediatric/adult and fetal small and large bowel as well as matching purified human gut epithelium. Furthermore, organoids were subjected to in vitro differentiation and genome editing using CRISPR/Cas9 technology.

Results

We discovered stable epigenetic signatures which define regional differences in gut epithelial function, including induction of segment-specific genes during cellular differentiation. Established

SUBMITTER: Kraiczy J 

PROVIDER: S-EPMC6839835 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature