Bacterial diversity in intestinal mucosa of antibiotic-associated diarrhea mice.

ABSTRACT: To probe into the mechanism of antibiotic-associated diarrhea (AAD), the bacterial diversity and composition in the intestinal mucosa of AAD mice were investigated. Twelve specific pathogen-free Kunming mice were divided into control group and model group. The mouse model of AAD was established by gavaging with antibiotics (mixture of gentamycin sulfate and cefradine) at a total dose of 23.33 ml kg^-1 day^-1 for 5 days continuously, twice a day. The mice in the control group were given with an equal amount of sterile water. Then, the intestinal mucosa DNA was extracted for 16S rRNA gene sequence analysis by high-throughput sequencing. The results showed that the alpha diversity of the two groups did not differ significantly from each other, while the composition of inte

SUBMITTER: Xie G 

PROVIDER: S-EPMC6842370 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature