T-cell phenotypes are associated with serum IgE levels in Amish and Hutterite children.

ABSTRACT:

Objectives

Amish children raised on traditional farms have lower atopy and asthma risk than Hutterite children raised on modern farms. In our previous study we established that the Amish environment affects the innate immune response to decrease asthma and atopy risk. Here we investigated T-cell phenotypes in the same Amish and Hutterite children as in our earlier study to elucidate how this altered innate immunity affects adaptive T cells.

Methods

Blood was collected from 30 Amish and 30 Hutterite age- and sex-matched children; cells were cryopreserved until analysis. Flow cytometry was used to analyze cell subsets. Atopy was determined based on allergen-specific and total IgE levels.

Results

Children exposed to Amish farms had increased activated regulatory CD4

SUBMITTER: Hrusch CL 

PROVIDER: S-EPMC6842432 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature