Association of dengue virus-specific polyfunctional T-cell responses with clinical disease severity in acute dengue infection.

ABSTRACT:

Introduction

Although the role of dengue virus (DENV)-specific T cells in the pathogenesis of acute dengue infection is emerging, the functionality of virus-specific T cells associated with milder clinical disease has not been well studied. We sought to investigate how the functionality of DENV-NS3 and DENV-NS5 protein-specific T cells differ in patients with dengue fever (DF) and dengue hemorrhagic fever (DHF).

Methods

Using intracellular cytokine assays, we assessed the production of interferon γ (IFNγ), tumor necrosis factor-α (TNF-α), macrophage inflammatory protein-1β (MIP-1β), and CD107a expression in adult patients with acute DF (n = 21) and DHF (n = 22).

Results

Quadruple cytokine-producing, polyfunctional DENV-NS3- and DENV-NS5-specific T cells were more freq

SUBMITTER: Wijeratne DT 

PROVIDER: S-EPMC6842812 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature