Phosphoproteomic identification of vasopressin V2 receptor-dependent signaling in the renal collecting duct.

ABSTRACT: Vasopressin controls water balance largely through PKA-dependent effects to regulate the collecting duct water channel aquaporin-2 (AQP2). Although considerable information has accrued regarding the regulation of water and solute transport in collecting duct cells, information is sparse regarding the signaling connections between PKA and transport responses. Here, we exploited recent advancements in protein mass spectrometry to perform a comprehensive, multiple-replicate analysis of changes in the phosphoproteome of native rat inner medullary collecting duct cells in response to the vasopressin V2 receptor-selective agonist 1-desamino-8D-arginine vasopressin. Of the 10,738 phosphopeptides quantified, only 156 phosphopeptides were significantly increased in abundance, and only 63 phosphopep

SUBMITTER: Deshpande V 

PROVIDER: S-EPMC6843035 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature