Review, Evaluation, and Directions for Gene-Targeted Assembly for Ecological Analyses of Metagenomes.

ABSTRACT: Shotgun metagenomics has greatly advanced our understanding of microbial communities over the last decade. Metagenomic analyses often include assembly and genome binning, computationally daunting tasks especially for big data from complex environments such as soil and sediments. In many studies, however, only a subset of genes and pathways involved in specific functions are of interest; thus, it is not necessary to attempt global assembly. In addition, methods that target genes can be computationally more efficient and produce more accurate assembly by leveraging rich databases, especially for those genes that are of broad interest such as those involved in biogeochemical cycles, biodegradation, and antibiotic resistance or used as phylogenetic markers. Here, we review six gene-targeted as

SUBMITTER: Guo J 

PROVIDER: S-EPMC6843070 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature