Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes.

ABSTRACT:

Background

Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting efforts to find new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as Escherichia coli, cold-adapted enzymes often form inactive aggregates. Therefore it is necessary to develop new low-temperature expression systems, including identification of new host organisms and complementary genetic tools. Psychrophilic bacteria, including Pseudoalteromonas haloplanktis, Shewanella and Rhodococcus erythropolis have all been explored as candidates for such applications. However to date none of these have found widespread use as effic

SUBMITTER: Soderberg JJ 

PROVIDER: S-EPMC6844050 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature