A cell-based screening system for RNA polymerase I inhibitors.

ABSTRACT: RNA polymerase I (RNA Pol I) is a "factory" that orchestrates the transcription of ribosomal RNA for constructing ribosomes as a primary workshop for protein translation to sustain cell growth. The deregulation of RNA Pol I often causes uncontrolled cell proliferation, leading to cancer. Efficient and reliable methods are needed for the identification of selective inhibitors of RNA Pol I. Yeast (Saccharomyces cerevisiae) is eukaryotic and represents a valuable model system to study RNA Pol I, especially with the availability of the X-ray crystal structure of the yeast homologue of RNA Pol I, offering a structural basis to selectively target this transcriptional machinery. Herein, we developed a cell-based screening strategy by establishing a stable yeast cell line with a stably inte

SUBMITTER: Tan X 

PROVIDER: S-EPMC6844272 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature