Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development.

ABSTRACT: Extracellular vesicles (EVs) have great potential as a source for clinically relevant biomarkers since they can be readily isolated from biofluids and carry microRNA (miRNA), mRNA, and proteins that can reflect disease status. However, the biological and technical variability of EV content is unknown making comparisons between healthy subjects and patients difficult to interpret. In this study, we sought to establish a laboratory and bioinformatics analysis pipeline to analyse the small RNA content within EVs from patient serum that could serve as biomarkers and to assess the biological and technical variability of EV RNA content in healthy individuals. We sequenced EV small RNA from multiple individuals (biological replicates) and sequenced multiple replicates per individual (technical re

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC6844434 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature