Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro.

ABSTRACT: Cell sheet technology is a novel tissue engineering technology that has been rapidly developed in recent years. As a novel technology, cell sheet technology is expected to become one of the preferred methods for cell transplantation. The present study investigated the biological effects of rutin on the formation of periodontal ligament stem cell (PDLSC) sheets and their resultant osteogenic properties. The results of Cell Counting Kit‑8 (CCK‑8) assay demonstrated that a concentration of 1x10‑6 mol/l rutin promoted the proliferation of PDLSCs more effectively compared with other designed concentrations. Rutin‑modified cell sheets could be induced by complete medium supplemented with 20 µg/ml vitamin C (VC) and 1x10‑6 mol/l rutin. Rutin‑modified cell sheets appeared thicker and more compact

SUBMITTER: Zhao B 

PROVIDER: S-EPMC6844602 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature