Structural basis for 2'-deoxyguanosine recognition by the 2'-dG-II class of riboswitches.

ABSTRACT: A recent bioinformatic analysis of well-characterized classes of riboswitches uncovered subgroups unable to bind to the regulatory molecule of the parental class. Within the guanine/adenine class, seven groups of RNAs were identified that deviate from the consensus sequence at one or more of three positions directly involved purine nucleobase recognition, one of which was validated as a second class of 2'-deoxyguanosine riboswitch (called 2'-dG-II). To understand how 2'-dG-II riboswitches recognize their cognate ligand and how they differ from a previously identified class of 2'-deoxyguanosine binding riboswitches, we have solved the crystal structure of a 2'-dG-II aptamer domain bound to 2'-deoxyguanosine. This structure reveals a global architecture similar to other members of the purine

SUBMITTER: Matyjasik MM 

PROVIDER: S-EPMC6847200 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature