Improved annotation of protein-coding genes boundaries in metazoan mitochondrial genomes.

ABSTRACT: With the rapid increase of sequenced metazoan mitochondrial genomes, a detailed manual annotation is becoming more and more infeasible. While it is easy to identify the approximate location of protein-coding genes within mitogenomes, the peculiar processing of mitochondrial transcripts, however, makes the determination of precise gene boundaries a surprisingly difficult problem. We have analyzed the properties of annotated start and stop codon positions in detail, and use the inferred patterns to devise a new method for predicting gene boundaries in de novo annotations. Our method benefits from empirically observed prevalances of start/stop codons and gene lengths, and considers the dependence of these features on variations of genetic codes. Albeit not being perfect, our new approach yiel

SUBMITTER: Donath A 

PROVIDER: S-EPMC6847864 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature