Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies.

ABSTRACT: To become fertile, mammalian sperm must undergo a series of biochemical and physiological changes known as capacitation. These changes involve crosstalk between metabolic and signaling pathways and can be recapitulated in vitro. In this work, sperm were incubated in the absence of exogenous nutrients (starved) until they were no longer able to move. Once immotile, energy substrates were added back to the media and sperm motility was rescued. Following rescue, a significantly higher percentage of starved sperm attained hyperactivated motility and displayed increased ability to fertilize in vitro when compared with sperm persistently incubated in standard capacitation media. Remarkably, the effects of this treatment continue beyond fertilization as starved and rescued sperm pro

SUBMITTER: Navarrete FA 

PROVIDER: S-EPMC6848031 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature