ITRAQ-based proteomics analysis of aqueous humor in patients with dry age-related macular degeneration.

ABSTRACT:

Aim

To preliminarily test proteomics in aqueous humor in patients with dry age-related macular degeneration (AMD) by using the proteomic technology.

Methods

Aqueous humor samples were collected from patients with or without dry AMD, who underwent cataract surgery. The aqueous samples were analyzed with isobaric tags for relative and absolute quantification (iTRAQ) combined with liquid chromatography tandem mass spectrometry (LC-MS/MS) technology. The differential expressed proteins were analyzed with gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) network analysis. The data were partly validated by ELISA and Western blot. False discovery rate (FDR) was used for statistical analysis.

Results

A total of

SUBMITTER: Qu SC 

PROVIDER: S-EPMC6848880 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature