Improvements of Microcontact Printing for Micropatterned Cell Growth by Contrast Enhancement.

ABSTRACT: Patterned neuronal cell cultures are important tools for investigating neuronal signal integration, network function, and cell-substrate interactions. Because of the variable nature of neuronal cells, the widely used coating method of microcontact printing is in constant need of improvements and adaptations depending on the pattern, cell type, and coating solutions available for a certain experimental system. In this work, we report on three approaches to modify microcontact printing on borosilicate glass surfaces, which we evaluate with contact angle measurements and by determining the quality of patterned neuronal growth. Although background toxification with manganese salt does not result in the desired pattern enhancement, a simple heat treatment of the glass substrates leads to improv

SUBMITTER: Hondrich TJJ 

PROVIDER: S-EPMC6848919 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature