Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis.

ABSTRACT:

Background

Corynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. However, the yield of coenzyme A needs to be improved, and the available constitutive promoters are rather limited in this strain.

Results

In this study, 20 putative DNA promoters derived from genes with high transcription levels and 6 promoters from molecular chaperone genes were identified. To evaluate the activity of each promoter, red fluorescence protein (RFP) was used as a reporter. We successfully isolated a range of promoters with different activity levels, and among these a fragment derived from the upstream sequence of the 50S ribosomal protein L2

SUBMITTER: Hou Y 

PROVIDER: S-EPMC6849255 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature