Tunable Riboregulator Switches for Post-transcriptional Control of Gene Expression.

ABSTRACT: Until recently, engineering strategies for altering gene expression have focused on transcription control using strong inducible promoters or one of several methods to knock down wasteful genes. Recently, synthetic riboregulators have been developed for translational regulation of gene expression. Here, we report a new modular synthetic riboregulator class that has the potential to finely tune protein expression and independently control the concentration of each enzyme in an engineered metabolic pathway. This development is important because the most straightforward approach to altering the flux through a particular metabolic step is to increase or decrease the concentration of the enzyme. Our design includes a cis-repressor at the 5' end of the mRNA that forms a stem-loop helix, occludin

SUBMITTER: Krishnamurthy M 

PROVIDER: S-EPMC6849397 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature