Automated noninvasive epithelial cell counting in phase contrast microscopy images with automated parameter selection.

ABSTRACT: Cell counting is commonly used to determine proliferation rates in cell cultures and for adherent cells it is often a 'destructive' process requiring disruption of the cell monolayer resulting in the inability to follow cell growth longitudinally. This process is time consuming and utilises significant resource. In this study a relatively inexpensive, rapid and widely applicable phase contrast microscopy-based technique has been developed that emulates the contrast changes taking place when bright field microscope images of epithelial cell cultures are defocused. Processing of the resulting images produces an image that can be segmented using a global threshold; the number of cells is then deduced from the number of segmented regions and these cell counts can be used to generate growth cur

SUBMITTER: Flight R 

PROVIDER: S-EPMC6849568 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature