Three-dimensional architecture of common benign and precancerous prostate epithelial lesions.

ABSTRACT:

Aims

Many glandular lesions can mimic prostate cancer microscopically, including atrophic glands, adenosis and prostatic intraepithelial neoplasia. While the characteristic histopathological and immunohistochemical features of these lesions have been well established, little is known about their three-dimensional architecture. Our objective was to evaluate the three-dimensional organisation of common prostate epithelial lesions.

Methods and results

500 μm-thick punches (n = 42) were taken from radical prostatectomy specimens, and stained with antibodies targeting keratin 8-18 and keratin 5 for identification of luminal and basal cells, respectively. Tissue samples were optically cleared in benzyl alcohol:benzyl benzoate and imaged using a confocal laser scanning microscope.

SUBMITTER: Verhoef EI 

PROVIDER: S-EPMC6849837 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature