Investigation of human apoB48 metabolism using a new, integrated non-steady-state model of apoB48 and apoB100 kinetics.

ABSTRACT:

Background

Triglyceride-rich lipoproteins and their remnants have emerged as major risk factors for cardiovascular disease. New experimental approaches are required that permit simultaneous investigation of the dynamics of chylomicrons (CM) and apoB48 metabolism and of apoB100 in very low-density lipoproteins (VLDL).

Methods

Mass spectrometric techniques were used to determine the masses and tracer enrichments of apoB48 in the CM, VLDL₁ and VLDL₂ density intervals. An integrated non-steady-state multicompartmental model was constructed to describe the metabolism of apoB48- and apoB100-containing lipoproteins following a fat-rich meal, as well as during prolonged fasting.

Results

The kinetic model described the metabolism of apoB48 in CM, VLDL

SUBMITTER: Bjornson E 

PROVIDER: S-EPMC6849847 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature