Reporter-based forward genetic screen to identify bundle sheath anatomy mutants in A. thaliana.

ABSTRACT: The evolution of C₄ photosynthesis proceeded stepwise with each small step increasing the fitness of the plant. An important pre-condition for the introduction of a functional C₄ cycle is the photosynthetic activation of the C₃ bundle sheath by increasing its volume and organelle number. Therefore, to engineer C₄ photosynthesis into existing C₃ crops, information about genes that control the bundle sheath cell size and organelle content is needed. However, very little information is known about the genes that could be manipulated to create a more C₄ -like bundle sheath. To this end, an ethylmethanesulfonate (EMS)-based forward genetic screen was established in the Brassicaceae C₃  species Arabidopsis thaliana. To

SUBMITTER: Doring F 

PROVIDER: S-EPMC6850095 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature