Screening of the antimycobacterial activity of novel lipophilic agents by the modified broth based method.

ABSTRACT: Most of the introduced susceptibility methods of Mycobacterium tuberculosis have some disadvantages for screening. Therefore, the selection of susceptibility assay for evaluating candidate agents must be determined case by case. In this study, we evaluated the validity of a modified broth dilution-based assay in comparison to the gold standard proportional method for microbial sensitivity test of new lipophilic compounds candidate as antitubercular agents. The in-vitro susceptibilities of 114 M. tuberculosis strains were separately tested against isoniazid and two lipophilic antitubercular agents (derivative of dihydropyridines) by employing the standard proportional method and a modified broth dilution-based assay. The results for isoniazid testing showed 100% concordance for sensitivity,

SUBMITTER: Zandhaghighi M 

PROVIDER: S-EPMC6850257 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature