Evaluation of a rapid and automated heparin-induced thrombocytopenia immunoassay.

ABSTRACT:

Introduction

Heparin-induced thrombocytopenia (HIT) is a potentially life-threatening adverse reaction of heparin. Laboratory evaluation of HIT is often not available within a reasonable time. We evaluated the HemosIL^® HIT-Ab_(PF4-H) (Instrumentation Laboratory), a rapid, on-demand, fully automated, latex immunoturbidimetric assay (LIA).

Materials and methods

Following determination of the LIA's reference interval and cutoff values, a multicenter study was conducted between March 2013 and June 2015. Plasma samples of HIT-suspected patients (n = 632) were collected and evaluated by LIA on the ACL TOP^® Family systems (Instrumentation Laboratory), enzyme-linked immunosorbent assays (EIA), and serotonin release assay (SRA). Patient characteristics

SUBMITTER: Refaai MA 

PROVIDER: S-EPMC6850659 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature