Antigen-specific CD8 T cells in cell cycle circulate in the blood after vaccination.

ABSTRACT: Although clonal expansion is a hallmark of adaptive immunity, the location(s) where antigen-responding T cells enter cell cycle and complete it have been poorly explored. This lack of knowledge stems partially from the limited experimental approaches available. By using Ki67 plus DNA staining and a novel strategy for flow cytometry analysis, we distinguished antigen-specific CD8 T cells in G₀ , in G₁ and in S-G₂ /M phases of cell cycle after intramuscular vaccination of BALB/c mice with antigen-expressing viral vectors. Antigen-specific cells in S-G₂ /M were present at early times after vaccination in lymph nodes (LNs), spleen and, surprisingly, also in the blood, which is an unexpected site for cycling of normal non-leukaemic cells. Most prolife

SUBMITTER: Simonetti S 

PROVIDER: S-EPMC6850756 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature