SWATH-based proteomics reveals processes associated with immune evasion and metastasis in poor prognosis colorectal tumours.

ABSTRACT: Newly emerged proteomic methodologies, particularly data-independent acquisition (DIA) analysis-related approaches, would improve current gene expression-based classifications of colorectal cancer (CRC). Therefore, this study was aimed to identify protein expression signatures using SWATH-MS DIA and targeted data extraction, to aid in the classification of molecular subtypes of CRC and advance in the diagnosis and development of new drugs. For this purpose, 40 human CRC samples and 7 samples of healthy tissue were subjected to proteomic and bioinformatic analysis. The proteomic analysis identified three different molecular CRC subtypes: P1, P2 and P3. Significantly, P3 subtype showed high agreement with the mesenchymal/stem-like subtype defined by gene expression signatures and characteriz

SUBMITTER: Lopez-Sanchez LM 

PROVIDER: S-EPMC6850959 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature