A microbial expression system for high-level production of scFv HIV-neutralizing antibody fragments in Escherichia coli.

ABSTRACT: Monoclonal antibodies (mABs) are of great biopharmaceutical importance for the diagnosis and treatment of diseases. However, their production in mammalian expression hosts usually requires extensive production times and is expensive. Escherichia coli has become a new platform for production of functional small antibody fragment variants. In this study, we have used a rhamnose-inducible expression system that allows precise control of protein expression levels. The system was first evaluated for the cytoplasmic production of super folder green fluorescence protein (sfGFP) in various production platforms and then for the periplasmic production of the anti-HIV single-chain variable antibody fragment (scFv) of PGT135. Anti-HIV broadly neutralizing antibodies, like PGT135, have potential for cl

SUBMITTER: Petrus MLC 

PROVIDER: S-EPMC6851033 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature