Identifying sex-linked markers in Litoria aurea: a novel approach to understanding sex chromosome evolution in an amphibian.

ABSTRACT: Few taxa exhibit the variability of sex-determining modes as amphibians. However, due to the presence of homomorphic sex chromosomes in many species, this phenomenon has been difficult to study. The Australian frog, Litoria aurea, has been relatively well studied over the past 20 years due to widespread declines largely attributable to chytrid fungus. However, it has been subject to few molecular studies and its mode of sex determination remained unknown. We applied DArTseq™ to develop sex-linked single nucleotide polymorphisms (SNPs) and restriction fragment presence/absence (PA) markers in 44 phenotypically sexed L. aurea individuals from the Molonglo River in NSW, Australia. We conclusively identified a male heterogametic (XX-XY) sex determination mode in this species, identifying 11 pe

SUBMITTER: Sopniewski J 

PROVIDER: S-EPMC6851140 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature