Escherichia coli Culture Filtrate Enhances the Growth of Gemmata spp.

ABSTRACT:

Background

Planctomycetes bacteria are known to be difficult to isolate, we hypothesized this may be due to missing iron compounds known to be important for other bacteria. We tested the growth-enhancement effect of complementing two standard media with Escherichia coli culture filtrate on two cultured strains of Gemmata spp. Also, the acquisition of iron by Gemmata spp. was evaluated by measuring various molecules involved in iron metabolism.

Materials and methods

Gemmata obscuriglobus and Gemmata massiliana were cultured in Caulobacter and Staley's medium supplemented or not with E. coli culture filtrate, likely containing siderophores and extracellular ferrireductases. We performed iron metabolism studies with FeSO₄, Fe

SUBMITTER: Kabore OD 

PROVIDER: S-EPMC6851166 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature