A novel approach to carotenoid accumulation in rice callus by mimicking the cauliflower Orange mutation via genome editing.

ABSTRACT:

Background

β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed that dominant expression of splicing variants in the Orange (Or) gene causes β-carotene accumulation in cauliflower curd. In this study, we focused on a putative orthologue of the cauliflower or gene in rice, Osor, and attempt to accumulate β-carotene in rice callus by modification of the Osor gene via genome editing using CRISPR/Cas9.

Findings

CRISPR/Cas9 vectors for the Osor gene were constructed and transformed into rice calli. Some transformed calli showed orange color due to β-carotene hyper-accumulation. Molecular analyses suggest that orange-colored c

SUBMITTER: Endo A 

PROVIDER: S-EPMC6851270 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature