Analyses of epithelial Na⁺ channel variants reveal that an extracellular β-ball domain critically regulates ENaC gating.

ABSTRACT: Epithelial Na⁺ channel (ENaC)-mediated Na⁺ transport has a key role in the regulation of extracellular fluid volume, blood pressure, and extracellular [K⁺]. Among the thousands of human ENaC variants, only a few exist whose functional consequences have been experimentally tested. Here, we used the Xenopus oocyte expression system to investigate the functional roles of four nonsynonymous human ENaC variants located within the β7-strand and its adjacent loop of the α-subunit extracellular β-ball domain. αR350Wβγ and αG355Rβγ channels exhibited 2.5- and 1.8-fold greater amiloride-sensitive currents than WT αβγ human ENaCs, respectively, whereas αV351Aβγ channels conducted significantly less current than WT. Currents in αH354Rβγ-expressing oocytes were s

SUBMITTER: Wang X 

PROVIDER: S-EPMC6851303 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature