A method of processing nasopharyngeal swabs to enable multiple testing.

ABSTRACT:

Objective

To develop a method to perform multiple tests on a single nasopharyngeal (NP) swab.

Methods

We collected a NP swab on children aged 2-12 years with acute sinusitis and processed it for bacterial culture, viruses, cytokine expression, and 16S ribosomal RNA gene sequencing analysis. During the course of the study, we expand the scope of evaluation to include RNA-sequencing, which we accomplished by cutting the tip of the swab.

Results

Of the 174 children enrolled, 126 (72.4%) had a positive bacterial culture and 121 (69.5%) tested positive for a virus. Cytokine measurement, as judged by adequate levels of a housekeeping enzyme (glyceraldehyde 3-phosphate dehydrogenase), appeared successful. From the samples used for 16S ribosomal sequencing we recovered, on av

SUBMITTER: Lopez SMC 

PROVIDER: S-EPMC6851467 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature