Dataset Information

Target highlights in CASP13: Experimental target structures through the eyes of their authors.

ABSTRACT: The functional and biological significance of selected CASP13 targets are described by the authors of the structures. The structural biologists discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP13 experiment.

SUBMITTER: Lepore R

PROVIDER: S-EPMC6851490 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Target highlights in CASP13: Experimental target structures through the eyes of their authors.

Lepore Rosalba R Kryshtafovych Andriy A Alahuhta Markus M Veraszto Harshul A HA Bomble Yannick J YJ Bufton Joshua C JC Bullock Alex N AN Caba Cody C Cao Hongnan H Davies Owen R OR Desfosses Ambroise A Dunne Matthew M Fidelis Krzysztof K Goulding Celia W CW Gurusaran Manickam M Gutsche Irina I Harding Christopher J CJ Hartmann Marcus D MD Hayes Christopher S CS Joachimiak Andrzej A Leiman Petr G PG Loppnau Peter P Lovering Andrew L AL Lunin Vladimir V VV Michalska Karolina K Mir-Sanchis Ignacio I Mitra A K AK Moult John J Phillips George N GN Pinkas Daniel M DM Rice Phoebe A PA Tong Yufeng Y Topf Maya M Walton Jonathan D JD Schwede Torsten T

Proteins 20190909 12

The functional and biological significance of selected CASP13 targets are described by the authors of the structures. The structural biologists discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP13 experiment. ...[more]

PMID: 31442339

Similar Datasets

Project description:While multiplexed fluorescence imaging is frequently used for in vitro microscopy, extending the technique to whole animal imaging in vivo has remained challenging due to the attenuation and scattering of visible and traditional near infrared (NIR-I) wavelengths. Fluorescence imaging using short-wave infrared (SWIR, 1000 - 1700 nm, a.k.a. NIR-II) light enables deeper tissue penetration for preclinical imaging compared to previous methods due to reduced tissue scattering and minimal background autofluorescence in this optical window. Combining NIR-I excitation wavelengths with multiple distinct SWIR emission peaks presents a tremendous opportunity to distinguish multiple fluorophores with high precision for non-invasive, multiplexed anatomical imaging in small animal models. SWIR-emitting semiconductor quantum dots (QDs) with tunable emission peaks and optical stability have emerged as powerful contrast agents, but SWIR imaging demonstrations have yet to move beyond two-color imaging schemes. In this study, we engineered a set of three high quantum yield lead sulfide/cadmium sulfide (PbS/CdS) core/shell QDs with distinct SWIR emissions ranging from 1100 - 1550 nm and utilize these for simultaneous three-color imaging in mice. We first use QDs to non-invasively track lymphatic drainage, highlighting the detailed network of lymphatic vessels with high-resolution with a widefield imaging over a 2 hr period. We then perform multiplexed imaging with all three QDs to distinctly visualize the lymphatic system and spatially overlapping vasculature network. This work establishes optimized SWIR QDs for next-generation multiplexed preclinical imaging, moving beyond the capability of previous dual-labeling techniques. The capacity to discriminate several fluorescent labels through non-invasive NIR-I excitation and SWIR detection unlocks numerous opportunities for studies of disease progression, drug biodistribution, and cell trafficking dynamics in living organisms.

Dataset Information

Target highlights in CASP13: Experimental target structures through the eyes of their authors.

Publications

Target highlights in CASP13: Experimental target structures through the eyes of their authors.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets