ANCHOR: a 16S rRNA gene amplicon pipeline for microbial analysis of multiple environmental samples.

ABSTRACT: Analysis of 16S ribosomal RNA (rRNA) gene amplification data for microbial barcoding can be inaccurate across complex environmental samples. A method, ANCHOR, is presented and designed for improved species-level microbial identification using paired-end sequences directly, multiple high-complexity samples and multiple reference databases. A standard operating procedure (SOP) is reported alongside benchmarking against artificial, single sample and replicated mock data sets. The method is then directly tested using a real-world data set from surface swabs of the International Space Station (ISS). Simple mock community analysis identified 100% of the expected species and 99% of expected gene copy variants (100% identical). A replicated mock community revealed similar or better numbers of expe

SUBMITTER: Gonzalez E 

PROVIDER: S-EPMC6851558 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature