Dataset Information

Application of Volatile Organic Compound Analysis in a Nutritional Intervention Study: Differential Responses during Five Hours Following Consumption of a High- and a Low-Fat Dairy Drink.

ABSTRACT:

Scope

Exhaled volatile organic compounds (VOCs) are a possible relevant target for noninvasive assessment of metabolic responses. Using a breathomics approach, it is aimed to explore whether lipid intake influences VOC profiles in exhaled air, and to obtain insight in intra- and interindividual variations.

Methods and results

Three human interventions are performed. In the first, 12 males consume a high-fat drink on three study days. In the second, 12 males receive a high- and a low-fat drink on 6 days. In the third, three volunteers consume the high-fat drink again for tentative compound identification. Participants are asked to exhale, for 5 h postprandial with 15-20 min intervals, into a proton-transfer-reaction mass spectrometer, and VOCs in exhaled air are measured. Consumption of a drink alters the VOC profile, with considerable interindividual variation and quantitative intraindividual differences between days. Consumption of two different drinks results in a distinct VOC profile, caused by several specific m/z values. Most of these compounds are identified as being related to ketone body formation and lipid oxidation, showing an increase in high- versus low-fat drink.

Conclusion

Exhaled VOCs have the potential to assess differences in metabolic responses induced by nutrition, especially when day-to-day variation can be minimized.

SUBMITTER: Hageman JHJ

PROVIDER: S-EPMC6852046 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Application of Volatile Organic Compound Analysis in a Nutritional Intervention Study: Differential Responses during Five Hours Following Consumption of a High- and a Low-Fat Dairy Drink.

Hageman Jeske H J JHJ Nieuwenhuizen Arie G AG van Ruth Saskia M SM Hageman Jos A JA Keijer Jaap J

Molecular nutrition & food research 20190805 20

<h4>Scope</h4>Exhaled volatile organic compounds (VOCs) are a possible relevant target for noninvasive assessment of metabolic responses. Using a breathomics approach, it is aimed to explore whether lipid intake influences VOC profiles in exhaled air, and to obtain insight in intra- and interindividual variations.<h4>Methods and results</h4>Three human interventions are performed. In the first, 12 males consume a high-fat drink on three study days. In the second, 12 males receive a high- and a l ...[more]

PMID: 31327167

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Project description:Drought stress is a major constraint on crop production. While Trichoderma fungi are widely recognized for enhancing plant tolerance to abiotic stresses, the fungal molecules responsible for these protective effects remain poorly defined. In this study, we investigated the role of 6-pentyl-a-pyrone (6PP), a volatile compound produced by diverse Trichoderma spp., in modulating plant responses to water deficit. Dwarf bean (Phaseolus vulgaris) seedlings grown from seeds coated with a carrier polymer delivering 6PP were significantly more tolerant to drought stress than seedlings from seeds coated with the polymer alone (control), exhibiting increased leaf area, root length, chlorophyll content, and photosynthetic rate. To elucidate the mechanisms induced by this molecule, comparative transcriptome analyses were performed in the presence and absence of 6PP under both wellwatered and drought conditions; only 49 DEGs were detected under wellwatered conditions. In contrast, 476 expressed genes were identified in drought-stressed plants grown from 6PP-coated seeds, indicating substantial transcriptional reprogramming. 6PP treatment reprogrammed stress-responsive gene networks, including pathways associated with photosynthesis, carbohydrate metabolism, phosphorylation-mediated signaling, defense responses, and oxidative stress regulation. In addition, drought-stressed plants treated with 6PP exhibited enhanced MAPK phosphorylation, providing protein-level evidence for activation of phosphorylation-mediated signaling pathways associated with stress tolerance. Functional enrichment analyses further highlight secondary metabolite pathways, including those for phenylpropanoid and flavonoid biosynthesis, suggesting enhanced production of protective compounds and maintenance of photosynthetic activity under drought stress. Several stress-related genes were modulated prior to drought exposure, indicating a possible priming effect. Collectively, our findings identify 6PP as a potential signaling molecule contributing to enhanced drought resilience and provide new insights into the molecular basis of Trichoderma-mediated stress protection.

Dataset Information

Application of Volatile Organic Compound Analysis in a Nutritional Intervention Study: Differential Responses during Five Hours Following Consumption of a High- and a Low-Fat Dairy Drink.

Scope

Methods and results

Conclusion

Publications

Application of Volatile Organic Compound Analysis in a Nutritional Intervention Study: Differential Responses during Five Hours Following Consumption of a High- and a Low-Fat Dairy Drink.

Similar Datasets

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