Evaluation of fecal microRNA stability in healthy cats.

ABSTRACT:

Background

Gastrointestinal (GI) cancer accounts for 14% of feline malignancies. There is a great need for reliable noninvasive diagnostic biomarkers to reach a timely diagnosis and initiate treatment. Fecal microRNAs (miRNAs) could be such a biomarker and have shown great potential in colorectal screening in people but have yet to be investigated in cats.

Objectives

We aimed to evaluate the presence and stability of feline fecal miRNA under different storage conditions (room temperature [RT], 4, and -20°C) and to evaluate the expression levels of specific fecal miRNAs collected on three separate days (days 1, 4, and 7) in healthy cats.

Methods

Healthy cats were prospectively recruited. Fecal samples were collected, aliquoted, and stored for 24 hours at RT and then tr

SUBMITTER: Lyngby JG 

PROVIDER: S-EPMC6852515 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature