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The Incorporation of Extracellular Vesicles from Mesenchymal Stromal Cells Into CD34⁺ Cells Increases Their Clonogenic Capacity and Bone Marrow Lodging Ability.

ABSTRACT: Mesenchymal stromal cells (MSC) may exert their functions by the release of extracellular vesicles (EV). Our aim was to analyze changes induced in CD34⁺ cells after the incorporation of MSC-EV. MSC-EV were characterized by flow cytometry (FC), Western blot, electron microscopy, and nanoparticle tracking analysis. EV incorporation into CD34⁺ cells was confirmed by FC and confocal microscopy, and then reverse transcription polymerase chain reaction and arrays were performed in modified CD34⁺ cells. Apoptosis and cell cycle were also evaluated by FC, phosphorylation of signal activator of transcription 5 (STAT5) by WES Simple, and clonal growth by clonogenic assays. Human engraftment was analyzed 4 weeks after CD34⁺ cell transplantation in nonobese

SUBMITTER: Preciado S

PROVIDER: S-EPMC6852558 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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The Incorporation of Extracellular Vesicles from Mesenchymal Stromal Cells Into CD34⁺ Cells Increases Their Clonogenic Capacity and Bone Marrow Lodging Ability.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

The Incorporation of Extracellular Vesicles from Mesenchymal Stromal Cells Into CD34+ Cells Increases Their Clonogenic Capacity and Bone Marrow Lodging Ability.

OmicsDI is part of the ELIXIR infrastructure

Tweets

The Incorporation of Extracellular Vesicles from Mesenchymal Stromal Cells Into CD34⁺ Cells Increases Their Clonogenic Capacity and Bone Marrow Lodging Ability.