MicroRNA-96-3p promotes metastasis of papillary thyroid cancer through targeting SDHB.

ABSTRACT:

Background

MicroRNA (MiRNA) is a small non-coding RNA which is implicated in a cohort of biological function in cancer, including proliferation, metastasis, apoptosis and invasion. MiR-96 has been reported to be involved in many cancers, including papillary thyroid cancer. However, the role of miR-96-3p in papillary thyroid cancer metastasis is still unclear.

Methods

qRT-PCR is used to detect the level of miR-96-3p and mRNA of SDHB in PTC tissues and cell lines. Western blot assays are used to verify the protein expression of SDHB. The transwell assays are performed to identify the migration ability of PTC cell lines. Moreover, dual-luciferase 3'-UTR reporter assays are chosen to illuminate the direct target of miR-96-3p.

Results

The relative miR-96-3p upregulate in P

SUBMITTER: Zhao X 

PROVIDER: S-EPMC6852711 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature