DNA methylation of the PLIN1 promoter downregulates expression in chicken lines.

ABSTRACT: Evidence suggests that Perilipin-1 (PLIN1) is subject to functional regulation by epigenetic modifications in women with obesity. However, whether chicken PLIN1 expression is regulated by DNA methylation is unknown. Here, Sequenom MassARRAY and real-time polymerase chain reaction (PCR) were conducted to analyze the promoter methylation status and expression of the PLIN1 gene in Northeast Agricultural University broiler lines divergently selected for abdominal fat content. We found that chicken PLIN1 expression was significantly higher in adipose tissue of fat-line broilers than in lean lines at 1-7 weeks of age, and was significantly positively correlated with abdominal fat percentage (AFP) in chicken adipose development (Pearson's r = 0.627 , P < 0.001 ). The

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6852845 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature