Genomic epidemiology supports multiple introductions and cryptic transmission of Zika virus in Colombia.

ABSTRACT:

Background

Colombia was the second most affected country during the American Zika virus (ZIKV) epidemic, with over 109,000 reported cases. Despite the scale of the outbreak, limited genomic sequence data were available from Colombia. We sought to sequence additional samples and use genomic epidemiology to describe ZIKV dynamics in Colombia.

Methods

We sequenced ZIKV genomes directly from clinical diagnostic specimens and infected Aedes aegypti samples selected to cover the temporal and geographic breadth of the Colombian outbreak. We performed phylogeographic analysis of these genomes, along with other publicly-available ZIKV genomes from the Americas, to estimate the frequency and timing of ZIKV introductions to Colombia.

Results

We attempted PCR amplification on 184

SUBMITTER: Black A 

PROVIDER: S-EPMC6852897 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature