Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation.

ABSTRACT: Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies because of their unique combination of two properties: pluripotency and a high proliferative capacity. To realize this potential, development of efficient hPSC differentiation protocols is required. In this work, sex-based differences are identified in a GSK3 inhibitor based endothelial progenitor differentiation protocol. While male hPSCs efficiently differentiate into CD34 ⁺CD31⁺ endothelial progenitors upon GSK3 inhibition, female hPSCs showed limited differentiation capacity using this protocol. Using VE-cadherin-GFP knockin reporter cells, female cells showed significantly increased differentiation efficiency when treated with VEGF during the second stage

SUBMITTER: Randolph LN 

PROVIDER: S-EPMC6853961 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature