Dysbiosis signatures of the microbial profile in tissue from bladder cancer.

ABSTRACT:

Background

To examine the microbial profiles in parenchyma tissues in bladder cancer.

Methods

Tissue samples of cancerous bladder mucosa were collected from patients diagnosed with bladder cancer (22 carcinoma tissues and 12 adjacent normal tissues). The V3-V4 region of the bacterial 16S rRNA gene was PCR amplified, followed by sequencing on an Illumina MiSeq platform. Bioinformatics analysis for microbial classification and functional assessment was performed to assess bladder microbiome diversity and variations.

Results

The predominant phylum in both tissues was Proteobacteria. The cancerous tissues exhibited lower species richness and diversity. Beta diversity significantly differed between the cancerous and normal tissues. Lower relative abundances of the microbia

SUBMITTER: Liu F 

PROVIDER: S-EPMC6854010 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature