High throughput genotyping of structural variations in a complex plant genome using an original Affymetrix® axiom® array.

ABSTRACT:

Background

Insertions/deletions (InDels) and more specifically presence/absence variations (PAVs) are pervasive in several species and have strong functional and phenotypic effect by removing or drastically modifying genes. Genotyping of such variants on large panels remains poorly addressed, while necessary for approaches such as association mapping or genomic selection.

Results

We have developed, as a proof of concept, a new high-throughput and affordable approach to genotype InDels. We first identified 141,000 InDels by aligning reads from the B73 line against the genome of three temperate maize inbred lines (F2, PH207, and C103) and reciprocally. Next, we designed an Affymetrix® Axiom® array to target these InDels, with a combination of probes selected at breakpoint site

SUBMITTER: Mabire C 

PROVIDER: S-EPMC6854671 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature