Diuron exposure and Akt overexpression promote glioma formation through DNA hypomethylation.

ABSTRACT: BACKGROUND:Diuron is an environmental component listed as a likely human carcinogen. Several other studies report that diuron can be oncogenic for bladder, urothelial, skin, and mammary cells. No study mentions the putative effect of diuron on the glioma occurrence. OBJECTIVES:We here wanted to investigate the effects of diuron exposure on the glioma occurrence while wishing to incriminate a putative implication of DNA methylation modulation in this process. METHODS:In in vivo model of glioma, diuron exposure was firstly compared or combined with oncogenic overexpressions already known to promote gliomagenesis. ELISA quantifying the 5-methylcytosine level on DNA was performed to examine the global DNA methylation level. Quantitative real-time polymerase chain reaction and proximity ligatio

SUBMITTER: Briand J 

PROVIDER: S-EPMC6854743 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature