M⁶A modification suppresses ocular melanoma through modulating HINT2 mRNA translation.

ABSTRACT:

Background

Dynamic N⁶-methyladenosine (m⁶A) RNA modification generated and erased by N⁶-methyltransferases and demethylases regulates gene expression, alternative splicing and cell fate. Ocular melanoma, comprising uveal melanoma (UM) and conjunctival melanoma (CM), is the most common primary eye tumor in adults and the 2nd most common melanoma. However, the functional role of m⁶A modification in ocular melanoma remains unclear.

Methods

m⁶A assays and survival analysis were used to explore decreased global m⁶A levels, indicating a late stage of ocular melanoma and a poor prognosis. Multiomic analysis of miCLIP-seq, RNA-seq and Label-free MS data revealed that m⁶A RNA modification posttranscriptional

SUBMITTER: Jia R 

PROVIDER: S-EPMC6854757 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature