Developing a high-performance liquid chromatography fast and accurate method for quantification of silibinin.

ABSTRACT: OBJECTIVE:Silibinin is an antioxidant agent and is shown to have anticancer effects in different cancers including lung, breast, colorectal, liver, prostate, and kidney. There are challenges in the clinical use of silibinin. The main limitation is low solubility, poor oral absorption, and extensive hepatic metabolism. We aim to develop a High-Performance Liquid Chromatography (HPLC) sensitive method for quantification of silibinin in aqueous samples to quantify its concentration in new formulations. A reverse-phase high-performance liquid chromatography (RP-HPLC) composed of C18 column as stationary phase and the mixture of methanol (90%) and water (10%) as mobile phase. The developed method was validated based on the established guidelines. RESULTS:The retention time for silibinin was see

SUBMITTER: Bakhshi F 

PROVIDER: S-EPMC6854794 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature