Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells.

ABSTRACT:

Introduction

Many pathological conditions may benefit from cell therapy using mesenchymal stromal cells, particularly from adipose tissue (ASCs). Cells may be grafted in an environment with a remnant polymicrobial component. The aim is to investigate the behavior of ASCs when brought in contact with a large panel of bacteria.

Materials and methods

Carboxyfluorescein-labelled bacterial interaction with ASCs was followed by confocal time-lapse microscopy. Costaining with LAMP-1 was also analyzed. Viability of 4 gram-negative and 4 gram-positive bacterial strains after 6 h of coculture with ASCs was assessed by agar colony counting and by flow cytometry using SYTO-62®/propidium iodide (PI) for membrane permeabilization and DiOC6 for depolarization. A murine model of periodontit

SUBMITTER: Monsarrat P 

PROVIDER: S-EPMC6855043 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature