Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells.

ABSTRACT: Transfusion of donor-derived red blood cells (RBC) is the most common form of cellular therapy. Donor availability and the potential risk of alloimmunization and other transfusion-related complications may, however, limit the availability of transfusion units, especially for chronically transfused patients. In vitro cultured, customizable RBC would negate these concerns and further increase precision medicine. Large-scale, cost-effective production depends on optimization of culture conditions. We developed a defined medium and adapted our protocols to good manufacturing practice (GMP) culture requirements, which reproducibly provided pure erythroid cultures from peripheral blood mononuclear cells without prior CD34+ isolation, and a 3 × 107-fold increase in erythroblasts in 25 days (or fr

SUBMITTER: Heshusius S 

PROVIDER: S-EPMC6855111 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature