Vapor Cannabis Exposure Promotes Genetic Plasticity in the Rat Hypothalamus.

ABSTRACT: It is well established that cannabis use promotes appetite. However, how cannabis interacts with the brain's appetite center, the hypothalamus, to stimulate feeding behavior is unknown. A growing body of evidence indicates that the hypothalamic transcriptome programs energy balance. Here, we tested the hypothesis that cannabis targets alternative polyadenylation (APA) sites within hypothalamic transcripts to regulate transcriptomic function. To do this, we used a novel cannabis vapor exposure model to characterize feeding in adult male Long Evans rats and aligned this behavioral response with APA events using a Whole Transcriptome Termini Sequencing (WTTS-Seq) approach as well as functional RNA abundance measurements with real-time quantitative polymerase chain reactions. We found that vap

SUBMITTER: Brutman JN 

PROVIDER: S-EPMC6856070 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature