Heterologous Expression of a Cryptic Gene Cluster from Streptomyces leeuwenhoekii C34^T Yields a Novel Lasso Peptide, Leepeptin.

ABSTRACT: Analysis of the genome sequence of Streptomyces leeuwenhoekii C34^T identified biosynthetic gene clusters (BGCs) for three different lasso peptides (Lp1, Lp2, and Lp3) which were not known to be made by the strain. Lasso peptides represent relatively new members of the RiPP (ribosomally synthesized and posttranslationally modified peptides) family of natural products and have not been extensively studied. Lp3, whose production could be detected in culture supernatants from S. leeuwenhoekii C34^T and after heterologous expression of its BGC in Streptomyces coelicolor, is identical to the previously characterized chaxapeptin. Lp1, whose production could not be detected or achieved heterologously, appears to be identical to a recently identified member

SUBMITTER: Gomez-Escribano JP 

PROVIDER: S-EPMC6856326 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature