Additive manufacturing of laminar flow cells for single-molecule experiments.

ABSTRACT: A microfluidic laminar flow cell (LFC) forms an indispensable component in single-molecule experiments, enabling different substances to be delivered directly to the point under observation and thereby tightly controlling the biochemical environment immediately surrounding single molecules. Despite substantial progress in the production of such components, the process remains relatively inefficient, inaccurate and time-consuming. Here we address challenges and limitations in the routines, materials and the designs that have been commonly employed in the field, and introduce a new generation of LFCs designed for single-molecule experiments and assembled using additive manufacturing. We present single- and multi-channel, as well as reservoir-based LFCs produced by 3D printing to perform sing

SUBMITTER: Ahmadi A 

PROVIDER: S-EPMC6856346 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature